Transparetn Exopolymer Particles (TEP) Production Trends of Two Marine Bacteria: Pseudidiomarina Homiensis and Pseudoalteromonas Atlantica

In this paper, biopolymer produced by marine bacteria was monitored over time to determine the TEP production coefficient. During the experiment, cultured marine bacteria was incubated in a 1 liter flask under room temperature with shaking. The cultured marine bacteria was allowed to grow in the flask for 7 days. Water samples were taken from the 1 liter flask to determine the TEP content and cell number in the samples. Furthermore, the samples were analyzed with LC-OCD to check the organic content at different growing stages as well. Cell number was determined via total cell counting on peri dishes with different dilution factors. The samples for TEP content determination was desalted via dialysis, and then measured with alcian blue staining method.